Stool sample for worms and eggs worm - indications for research

Currently, stool sample for worms a Routine laboratory diagnostic Manipulation, appointed, children, and adults, such as in the framework of the investigations to determine the causes of pathological symptoms, and in the course of the preventive annual medical examination. is This analysis, although the General distribution, not always the people understand, so his appointment to consider the methodology of the implementation and the decryption of the results.

Research on enterobiasis

Stool sample for worms (endless screw) – General characteristics

Stool sample for worms properly investigated as the analysis on eggs a worm, because in the course of performance of employee tampering diagnostic laboratory faeces for the presence of eggs of various parasitic worms, the crushed organs and tissues of the human organism. And as the collective term for all of the parasitic worms – these worms, the main objective of the analysis is the detection or confirmation of the absence of eggs data of parasites.

The fact that the various organs and tissues of the human body can parasitize about 250 species of worms (ribbon worms), which reproduce by eggs. These eggs get into the intestine and draw to the outside together with the feces. The study smear of feces under the microscope you can see the eye and the larvae and eggs of various parasitic worms, so that their presence is diagnosed. That is, the analysis Kala on eggs a worm is a method for the diagnosis of Helminthiasis – diseases caused by parasitic worms (worms).

What are the parasites (tape worms), you can identify with the help of the analysis Kala?

In the course of the currently used laboratory methods for the analysis Kala on eggs of the worm may be due to the presence of parasitic worms (helminths) will be:

The class of roundworms (nematodes):

  • Roundworm (Ascaris lumbricoides);
  • Whip-worm (Trichocephalus trichiurus);
  • Thominx aerofilus;
  • Ancylostoma duodenale;
  • American hookworm (Necator americanus);
  • Strongyloides stercoralis.

The class of flatworms (trematodes-flukes):

  • Liverwort liver (Fasciola hepatica);
  • Cat's dvuustka (Opisthorchis felineus);
  • Dicrocoelium lanceatum;
  • Schistosoma mansoni et japonicum.

Class tapeworms (cestody):

  • Tsepen unarmed (taenia rhynchus saginatus);
  • Tsepen armed (Taenia solium);
  • Broad tapeworm (Diphyllobothrium latum);
  • Tapeworm is small (Diphyllobothrium minus).
Diagnosis

That is to say, all of the above types of parasitic worms found in the usual analysis Kala on eggs a worm. It should be understood that in kalowych the masses, not even worms, and particles of their shells, or eggs are found, the deferred propagation. Accordingly, when the doctor looks in the feces eggs of some parasitic worms, then it means that you would only have worms, and it is, therefore, somehow got into the organism of the concrete person. That is, the presence of eggs of helminths in Calais, you can say with 100 % foreign security that the Person is infected, it is parasitic information worm, you can excrete from the body.

In addition to these worms on the analysis Kala on eggs a worm, the infection can protozoa to reveal, such as:

  • Dysenteric amoeba (Entamoeba histolytica);
  • Giardia (Giardia Lamblia).

Also in the faeces can be found, cyclosporins and their larvae.

To pass if you need to, the analysis Kala on eggs a worm (certificate of research)

The analysis Kala on eggs a worm gets in two possible cases:

The suspected infection by helminths (Helminthiasis);

Barrier Analysis.

Barrier analysis is a mandatory investigation in the Routine procedures for the investigation of people on the work in connection with the sale, processing or storage of food, contacts with people and other fields of action, the transmission of worms, other. In this Situation, the analysis Kala on eggs a worm, a Screening, that is, the massive and rapid diagnosis of Helminthiasis in many people. Briefly, one can say that as a binding Barrier analysis of a feces on eggs a worm for the completion of the sanitary Laptop and entry to the Kindergarten, school, swimming pool or before a planned hospital stay in a hospital. This analysis should happen, because the human being is in contact with the other, but if the worm he provokes is infected, can infect others, the disease has spread.

The second indication for the implementation of the analysis Kala on eggs a worm is a suspected infection with any of these worms. Suspected worm infections (infection with parasitic worms) occurs when people of different VR-symptoms of disorders of the various organs in the absence of the corresponding disease. The fact that parasitic worms (helminths) can you beat the various organs and tissues, e.g. the intestine, the urinary bladder, the urethra, brain, liver, heart, lymph nodes, and other causes of the symptoms of various diseases. If the survey diseases undetectable disease of the organ, on the hand, there are pathological symptoms, the suspected worm is produced, of course, requires the confirmation or refutation. Exactly for the purpose of confirmation or refutation of Helminthiasis, the analysis Kala on eggs of the worm in such a Situation.

The preparation to the completion of the analysis of a feces on worms

The analysis Kala on eggs a worm shows for extradition, if the Person is any of the following symptoms:

  • Inflammatory diseases of the gastro-intestinal tract (e.g., abdominal pain, diarrhea, constipation, bloating, etc.);
  • Fatigue;
  • Irritability;
  • Disturbing Dream;
  • Bruxism (gritting the teeth);
  • Itching in the anal area;
  • Vulvovaginitis in women;
  • Infection of the urinary tract;
  • Growth failure and body weight in growing children and adolescents;
  • An increased number of eosinophils in the blood;
  • Allergic syndrome of unknown nature, when it is not possible to identify the Allergen, causes a pathological reaction on the part of the organism;
  • Frequent colds and viral infections.

If any of these symptoms be you need to pass the analysis Kala on eggs of the worm, as they are diseases, factors sufficient for the suspected worm. However, only the analysis Kala may not be enough for the diagnosis of Helminthiasis, and in the case of suspected parasites in samples of bile, Sputum, duodenal contents, and other biological mysteries of the human body, in which the eggs or larvae of parasitic worms.need to make

The preparation to the completion of the analysis of a feces on worms

Prior to the delivery of the Kala on eggs of the worm should be within a week of not taking castor oil or mineral oil, antibiotics, Anti -, and anti-parasitic agents as well as drugs of bismuth and Magnesium Also within 7 – 14 days prior to the delivery of the Kala on eggs a worm not irrigoscopy recommended.

In addition, you will need to adjust psychologically and morally, on what is a must-have for the quality of the diagnosis of Helminthiasis on one, but three or six samples of feces, will you donate a day or every 1 – 2 days. In General, you need three samples of feces, but if it is suspected dysentery amoeba or Giardia, best six specimens of faeces.

It is recommended that in advance, prepare the utensils for the samples of feces. In today's time, not the usual many people from childhood are boxes used to match, since the laboratory special plastic container with a lid. If in the laboratory, the Container is not given, you can buy it in the traditional pharmacy. This is better to use disposable containers, and not any existing home the dishes or cardboard, because they may be the larvae of the parasite or eggs, the accidentally of the dirty hands, the in the study be identified, and thus, the person a false positive result is given.

Laboratory

To produce no special preparation for the task of the analysis Kala on eggs a worm is not necessary.

How to collect properly the feces for the analysis on eggs a worm?

Prior to the bowel movements do not need to urinate, the urine came to the fecal mass. Then you don't have to my skin Anus, poop in the Fluid container, from where easily enter samples of feces (e.g., pot, basin, etc.).

To collect from the entire mass of feces small volume, suitable for about two teaspoons of tea, the experiments with different parts of the feces special Stick. If the container for excrement, then you can collect samples of common wood kitchen match or a stick. Optimal take 3 – 4 small pieces from the sides, the front and rear parts of the upper surface as well as in the interior of the feces. All of the collected part of the feces was closing in a sealing container and lid. On the Container, stick a piece of paper with the family name, the name and the name of the father, and quickly to the laboratory to be transported.

For the most accurate outcome in the best trial, Kala on eggs of the worm in the laboratory within 30 – 45 minutes after a bowel movement and your fence. If, within such a short period of time to submit a sample of faeces to the laboratory is not possible, then he can within a maximum of 5 – 8 hours, is necessarily subject to retention of feces in the refrigerator at a temperature of +4 to +8oC. When the samples of feces for longer than 60 minutes located, not in the fridge, but in a warm room, then they are not suitable for the implementation on the worm eggs. In this case, you have to throw spoiled sample and collect new after the next bowel movement.

For accurate and comprehensive diagnosis only give three samples of faeces must. And you have to collect the samples daily or 1 – 2 days. The result of the study can only try, according to the investigation of the three.

The result of the analysis Kala on eggs a worm and its decryption

In the standard, the result of the analysis Kala must be on the eggs of the worm in all three samples negative. This does not mean that the worm detected in eggs, and the person is not infected with parasitic worms.

A positive result in at least one of the samples of feces is an indication that a Person is discovered, the worms, and, therefore, is infected with parasitic worms. In such a Situation, you need a course of treatment and one month after completion of re stool sample to submit.

If the result of the analysis Kala on eggs a worm positive, which indicate what type of parasitic helminths was discovered. Exemplary formulation of a positive analysis Kala on eggs a worm as follows: "Positive, eggs of Ascaris". In each laboratory formulation may vary slightly, but in your audition, the term "positive" means the presence of parasitic worms, and the name of the species of worms, the eggs were found.

What are the methods to use the laboratory for the analysis Kala on eggs a worm?

The identification of parasitic worms in samples of feces in the laboratory is done with the help of the methods moprovskaja (helminthologia), i.e., the detection of eggs of helminths. In the laboratories for the diagnosis of parasites in humans in General, three main use-methods of moprovskaja :

    Container
  1. Microscopy-native smear;
  2. Method Of Full Born;
  3. The Method Of Telemann.

Usually attempt to Cala successively all the three methods moprovskaja . Wherein, in the first line of microscopy to produce native-smear, and then in any order make the attempt full born and Telemann.

Microscopy-native smear

Microscopy-native smear, a thorough examination under the microscope, small drops of faeces, blurred, a thin layer on the object carrier. First of all, on the glass 50% glycerol is applied with a drop, which facilitates the uniform distribution of the material. Then take the faeces, a small piece and mix it with distilled water until the state of sour cream. From the finished mass Cala take a small drop, place it on the glass and mixing stick of wood with glycerol. Then this magic wand to smear a drop on the surface of the glass a thin layer. Then smear a cover glass and look at the Material under the microscope.

Try Full Born

The try full born, consists in the mixing of sewage with salt water, the high-density and specific weight, allowing the worm eggs to pop-up on your surface. For carrying out the tests, 10 G of feces stirring with 100 ml of a 50% solution of cooking salt. The solution is prepared as follows – 450 – 500 G of salt dissolved in 1 Liter of water, heat it to a temperature of 50 – 70oC.

The mixture of feces with the salty water to a quiet place for 1 – 1.5 hours, the eggs of the worms appeared, on the surface of the solution. After the required number of time appeared on the surface of the foil is removed, looped and wear on the slide. The film, close the cover glass covers and under the microscope to study.

The Attempt By Telemann

Analysis

The attempt by Telemann is a mixture of feces with hydrochloric acid and ether, with which the worm eggs to give. For the production of samples by Telemann, a small amount of feces mixed with 3 – 4 ml of 30% hydrochloric acid and 15 – 20 ml of distilled water. Then the sediments of Cala paste mix 1 – 1.5 ml of Ether, and carefully close the stopper the vessel tightly, or the rubber cover. Then, the vessel will tilt several times in neck to the bottom, stir the contents. Thereafter, the slurry was filtered through a layer of gauze and centrifuged. Obtained after centrifugation the Sediment was close to the slides, cover glass and under the microscope to study.

If at least in an attempt to worm were found eggs, so the result of the analysis is positive.

Stool sample for worms – where to submit?

A chair to make sample for worms in each of the clinical-diagnostic laboratory in the local clinic or hospital. However, to achieve the maximum consistent result, a stool sample is recommended in specialized bacteriological laboratories, the sanitary-epidemiological services of the respective city or County.

In addition, the analysis passed, you can, in a private laboratory or the clinic, the license for such a diagnostic examination.

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01.06.2019